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Geet Gaata Chal (1975) Hindi Movie…
Geet Gaata Chal (1975) — Classic Devotional Hindi Song — Geet Gata.
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We show here that the hsp70, hsp90 and hsp70-2 proteins are not only inducible by heat shock in wild-type cells but are also inducible by various DNA-damaging agents in a series of Hoxb4-transformed cell lines. In contrast, such proteins are constitutively expressed in cells containing the neomycin resistance gene and transfected with the Hoxb4 gene. The response to alkylating agents was investigated in detail. The finding that Hoxb4-transformed cells are also hypersensitive to such agents prompted us to study the protein levels in these cells. The abundance of hsp70 and hsp90 was determined by gel electrophoresis and Western blot analysis. We found that a similar induction pattern was followed for both proteins. We also determined the size and abundance of hsp70-2 protein in wild-type and Hoxb4-transformed cells. The inducibility of hsp70-2 could not be detected in cells transformed with Hoxb4. As a control, we also analyzed the response of the transcription factor c-fos to alkylating agents in Hoxb4-transformed cells. The level of c-fos protein was similar in the two cell types. When induced by heat, c-fos was only present in Hoxb4-transformed cells but was absent in wild-type cells. Thus, we conclude that hsp70 and hsp90 in Hoxb4-transformed cells is not dependent on heat shock protein synthesis, and that Hoxb4 could contribute to this phenotype through the induction of one or more genes involved in the cellular response to DNA damage.Quake IV
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QuakeIV
QuakeIV is the fourth instalment in the Quake series of first-person shooters and was developed by id Software and released for the PC on June
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