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Objective: To investigate the relationship between single nucleotide polymorphisms (SNPs) of the apolipoprotein E (Apo E) gene and levels of lipid and apolipoprotein E in Chinese non-alcoholic fatty liver disease patients. Methods: A total of 210 patients with non-alcoholic fatty liver disease (NAFLD) were selected from the People’s Hospital of Liaocheng City. Apo E gene polymorphisms were determined by using the digestion products separated by using polyacrylamide gel electrophoresis (PAGE). Lipid and apolipoprotein E levels in the serum were measured using the method of colorimetry. Data were analyzed by ANOVA, Kruskal-Wallis H and chi-square test. Results: (1) The prevalence of alleles and genotypes of the Apo E4/4 genotype in NAFLD patients was 17.0% (35/210). The prevalence of alleles and genotypes of the Apo E3/3 genotype in NAFLD patients was 46.0% (95/210). (2) Levels of serum TG, TC, LDL-C and Apo B in Apo E4 carriers were higher than those in Apo E3 carriers (P<0.05). (3) Levels of serum TG, TC, LDL-C and Apo B in the NAFLD patients with Apo E4/4 genotype were significantly higher than those in the patients with Apo E3/3 or Apo E3/4 genotype (P<0.05). Conclusions: The prevalence of
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